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KMID : 0613820060160050834
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Journal of Life Science
2006 Volume.16 No. 5 p.834 ~ p.839
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Changes in Cell Wall Components, and Solubilization and Depolymerization of Pectin and Neutral Sugar Polymers during Softening of ‘Tsugaru’ Apples
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Choi Cheol
Kang In-Kyu
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Abstract
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This study was carried out to investigate changes in cell wall components and solubilization and depolymerization of pectin and neutral sugar polymers during softening of ‘Tsugaru’ apples. Pectic polysaccharides were solubilized in different solvents, distilled-water, 0.05 M CDTA, 0.05 M Na₂CO₃, and 8 M KOH, from cell wall materials during fruit softening. The uronic acid contents in distilled-water fraction rapidly increased along with fruit softening at 4 weeks after ambient storage. In the change of non-cellulosic neutral sugars in the cell wall of ‘Tsugaru’ fruits, the major sugar was galactose and arabinose in distilled-water, 0.05 M CDTA and 0.05 M Na₂CO₃ soluble fractions, and it was glucose, galactose and xylose in 8 M KOH fraction. Especially the change of galactose contents in distilled-water fraction was increased greatly along with fruit softening. When uronic acid polymers (UAP) and carbohydrate polymers (CP) in distilled-water fraction were filtered and separated using Sepharose CL-2B column, the high molecular UAP and CP were degraded to the low molecular ones from at harvest to softening fruit. Thus, the amount of high molecular polymers were greatly decreased along with fruit softening.
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KEYWORD
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Apple, cell wall, uronic acid, non-cellulosic neutral sugar, fruit softening
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